71 CHEMICALS KNOWLEDGE HUB Issue 2 / October 2025 LIFE SCIENCES therapies beyond infectious diseases to areas such as oncology, rare genetic disorders, and regenerative medicine. These technological breakthroughs have fueled the rapid expansion of the market, making mRNA one of the most promising platforms in modern medicine. One of the key technological advancements in mRNA synthesis is the improvement of in vitro transcription (IVT) techniques, which have led to higher yields and purity of synthesized mRNA. IVT is the process used to synthesize mRNA by copying a DNA template, and recent improvements have increased the efficiency of this process, making large-scale mRNA production more feasible. For instance, in May 2023, TriLink BioTechnologies developed proprietary CleanCap technology, CleanCap M6 analog, which enhances the capping efficiency of mRNA, resulting in increased stability and translation efficiency of the mRNA in cells. In addition, modifications to mRNA molecules, such as the incorporation of pseudouridine or N1-methyl-pseudouridine, have reduced the immunogenicity of mRNA, meaning that the immune system is less likely to attack it as a foreign entity. This has been crucial in ensuring that mRNA-based therapies, such as vaccines, are effective and safe for repeated use. BioNTech and Moderna utilize modified mRNA in their COVID-19 vaccines, which enhances their safety profile by minimizing adverse immune reactions. This technological leap has been central to the success of these vaccines, enabling them to achieve emergency use authorization and wide distribution. mRNA synthesis & manufacturing market: ecosystem analysis Automation in mRNA synthesis has allowed companies to produce large quantities of mRNA in a shorter time frame while maintaining high levels of consistency and purity. Advanced sequencing technologies and bioinformatics tools have also contributed to the growth of mRNA synthesis by enhancing the precision and efficiency of mRNA design. Bioinformatics tools are used to design mRNA sequences with increased stability, reduced immunogenicity, and optimized translation into proteins within the body. One of the main factors propelling the growth of the mRNA synthesis & manufacturing market is the growing outsourcing of mRNA synthesis and production to contract development and manufacturing organizations (CDMOs). Through outsourcing, biotech, and
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